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. 2018 May 29;9:1164. doi: 10.3389/fmicb.2018.01164

FIGURE 2.

FIGURE 2

Lentinula edodes mycelia inhibits the early phases of influenza virus infection in vitro. (A) Madin-Darby canine kidney (MDCK) cells were infected with WSN at MOI = 3 in the absence or presence of 300 μg/ml of LEM. At 7 h post infection, total RNAs were purified and quantitatively analyzed by reverse transcription followed by real-time PCR with primers specific for segment 5 viral genome RNA and NP mRNA. The results are averages from three independent experiments with standard deviations, and the statistical significance was determined by Student’s t-test. (B) 293T cells were transfected with plasmids encoding PA, PB1, PB2, and NP genes and a plasmid for the expression of artificial influenza virus genome encoding luciferase gene as a reporter in the presence or absence of 300 μg/ml of LEM. The results are averages from three independent experiments with standard deviations, and the statistical significance was determined by Student’s t-test.