Table 1.
Strain list used in this study.
| Strain | Genotype or plasmid | Source |
|---|---|---|
| E. coli | ||
| DH5α | F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK-mK+), λ- | Hanahan, 1985 |
| DH5α | pAD, UAS1B16TEF promoter | Mirończuk et al., 2017 |
| DH5α | pAD-EYI1, for overexpression of YALI0F01628g | This study |
| DH5α | pΔEYI1, for deletion of YALI0F01628g | This study |
| DH5 α | pΔku70, for deletion of Ylku70 | This study |
| DH5 α | pΔku80, for deletion of Ylku80 | This study |
| Y. lipolytica | ||
| A101 | Wild-type strain | Rzechonek et al., 2017 |
| Wratislavia K1 | Spontaneous mutant strain | Rymowicz et al., 2009 |
| AJD | MATA, A101,Δura | Mirończuk et al., 2016 |
| AJDD | MATA, AJD : ΔYlKU70, ΔYlKU780 | This study |
| AJDD pAD-EYI1 | MATA, AJDD, overexpression YALI0F01628g | This study |
| AJDD ΔEYI1 | MATA, AJDD Δ YALI0 F01628g | This study |
| AJDD c-EYI1 | MATA, AJDD complementation of YALI0F01628g | This study |