Figure 1.

Human Mesenchymal Stem Cells Were Subjected to Transformation in Mouse Liver Overexpressing P62

(A) Mouse athymic Balb/C mouse (a severe combined immunodeficiency mouse) liver in vivo transfection with pCMV6-AC-GFP, pCMV6-AC-GFP-P62, and pGFP-V-RS-P62 plasmids. The western blotting analysis with anti-P62 in mice liver tissue is shown. β-actin as internal control is shown. (B) The schematic illustrates that mouse mesenchymal stem cells, in which P62 was overexpressed or knocked down, were injected into the athymic Balb/C mouse liver capsule under the B ultrasound guide. Mice were fed with carbon tetrachloride (CCL₄) for three months. (C) The mice were stratified, the tumors recovered, and xenograft tumor photographed in the six groups as indicated in left. (D) The wet weight of each tumor for each mouse in pCMV6-AC-GFP-P62 plus CCL4 group. (E) The wet weight of each tumor was determined for each mouse. Each value was presented as mean ± SEM; **p < 0.01. (F) A portion of each tumor was fixed in 4% paraformaldehyde and embedded in paraffin for histological H&E staining. The representative analytic results of H&E are shown (100×).