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. 2018 Mar 9;11:367–381. doi: 10.1016/j.omtn.2018.03.002

Figure 7.

Figure 7

The Activated H-Ras Induced Some Gene Abnormal Expression of Specific Genes in the P62-Overexpressing or Knockdown Mesenchymal Stem Cells Treated with TNF-α

(A) GST pull-down using GST-Raf1-RBD system followed by western blotting anti-GTP Ras in the mesenchymal stem cells transfected with pCMV6-AC-GFP (GFP-control), pCMV6-AC-GFP-P62 (GFP-P62), pGFP-V-RS (RNAi control), and pGFP-V-RS-P62 (P62 RNAi). INPUT refers to western blotting with anti-H-Ras. (B) Western blotting with anti-pRaf, anti-Raf, anti-pMEKK1, anti-MEKK1, anti-pERK, anti-ERK, anti-pMEKK4, anti-MEKK4, anti-pMEK, anti-MEK, anti-pJak, anti-Jak, anti-pEIK, anti-EIK, anti-P38, anti-pPI3K, anti-PI3K, anti-pmTOR, or anti-pAKT in the mesenchymal stem cells transfected with pCMV6-AC-GFP (GFP-control), pCMV6-AC-GFP-P62 (GFP-P62), pGFP-V-RS (RNAi control), and pGFP-V-RS-P62 (P62 RNAi). β-actin served as an internal control.