Figure 8.

The Rescued Experiment of Carcinogenesis Effect of the P62 in TNF-α-Treated Mesenchymal Stem Cells Transfected with pCMV6-AC-P62 (GFP-P62), pCMV6-AC-GFP-P62 (GFP-P62) plus pcDNA3.1-H-Ras (Ras), pCMV6-AC-P62 (GFP-P62) plus TNF-α, and pCMV6-AC-P62 (GFP-P62) plus TNF-α plus pGFP-V-RS-H-Ras (Rasi)

(A) Western blotting analysis with anti-P62 and anti-H-Ras in mesenchymal stem cells. β-actin served as internal control. (B) Cells growth assay using CCK8. Each value was presented as mean ± SEM. (C) Cells soft-agar colony formation assay. (D) Tumorigenesis test in vivo in these mesenchymal stem cells. (a) The mice were stratified, and the tumors were recovered. (b) The wet weight of each tumor was determined for each mouse. Each value was presented as mean ± SEM. **p < 0.01; *p < 0.05.