Analysis of the Generated PCR Product, Synthetic hGLuc mRNA, and Cy3-Labeled mRNA, by 1% Agarose Gel Electrophoresis
(A) 200 ng hGLuc DNA (left) or hGLuc mRNA (right) was loaded on 1% agarose gel. The purity and the specific length of the PCR product and synthetic mRNA were confirmed by a single band at around 900 bases. (B) First, Cy3-labeled mRNA was detected using an UV transilluminator, and then all nucleic acids were detected by GelRed staining.