Figure 6.

Detection of Luciferase Activity after Microinjection of Synthetic hGLuc mRNA into Porcine Skin

(A) Luciferase activity was determined in medium (n = 4) and skin lysates (n = 5) 24, 48, and 72 hr after microinjection of 1.5 μg hGLuc mRNA complexed with Lipofectamine 2000 (L200) (B) Additionally, 1.5 μg hGLuc mRNA was injected without Lipofectamine 2000 complexation, referred to as naked mRNA, into the skin (n = 3). The medium was collected, and fresh medium was added every 24 hr. Luciferase activity was determined in the collected medium. Skin pieces injected with only medium or medium plus Lipofectamine 2000 served as controls. Results are presented as mean ± SEM. Statistical differences were determined using two-way ANOVA followed by Bonferroni’s multiple comparisons test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).