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. 2018 Jun 1;9:1134. doi: 10.3389/fmicb.2018.01134

FIGURE 2.

FIGURE 2

Amplification and analysis of the nifH gene of SnebYK. (A) Amplicon of SnebYK nifH (363 bp) in the left lane and DNA ladder in the right lane. (B) Transcriptional analysis of the SnebYK nifH gene in nitrogen-free medium determined with RT-PCR. The lane labeled “-N” shows the expression of the 16S rRNA and nifH gene of SnebYK grown in ACCC55 nitrogen-free medium; the lane labeled “+N” shows the expression of the 16S rRNA and nifH gene of SnebYK grown in nutrient agar medium. The transcript level of the 16S rRNA was used as a loading control; the transcript level of nifH of SnebYK grown in nutrient agar medium was used as a negative control. (C) Dendrogram based on the nifH sequences of SnebYK and other strains in the genus Klebsiella with similar nifH sequences. This analysis was performed using the neighbor-joining method in Mega 7.0.26 with a bootstrap value of n = 1000.