Figure 1.

miR372 Accelerates Liver Cancer Cell Growth In Vitro and In Vivo

(A) (a) The photography of the Hep3B cell lines infected with rLV or rLVmiR372. (b) Northern blotting analysis of miR372 in Hep3B cell lines infected with rLV and rLV-miR372 is shown. U6 as internal control is shown. (c) The real-time PCR detection of mature miR372 in Hep3B cell lines infected with rLV and rLV-miR372, respectively, is shown. Each value was presented as mean ± SEM. **p < 0.01. (B) Cell proliferation assay was performed in 96-well format using the CCK8 cells proliferation kit to determine the cell viability as described by the manufacturer. Each sample was assayed in triplicates for 3 days consecutively. Cell growth curve was based on the corresponding relative values of OD450, and each point represents the mean of three independent samples. Data are means of value from three independent experiments; mean ± SEM. **p < 0.01 ; *p < 0.05. (C) Cell BrdU assay is shown. Data are means of value from three independent experiments; mean ± SEM. **p < 0.01; *p < 0.05. (D) (a) The photography of colonies from the cell lines indicated in left is shown. (b) Cell plate colony formation ability assay is shown. Data are means of value from three independent experiments; mean ± SEM. **p < 0.01; *p < 0.05. (E) (a) The photography of the xenograft tumors from Balb/C-null mouse injected with Hep3B cells transfected with rLV and rLV-miR372 subcutaneously at armpit is shown. (b) The xenograft tumor weight (gram) in the two groups is shown. Data were means of value from six BALB/c mice; mean ± SEM; n = 6; *p < 0.05; **p < 0.01. (c) A portion of each xenograft tumor was fixed in 4% formaldehyde and embedded in paraffin, and the micrometers of sections (4 μm) were made for H&E staining (original magnification ×100).