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. 2018 Apr 12;11:494–507. doi: 10.1016/j.omtn.2018.04.001

Figure 2.

Figure 2

miR372 Promotes CTCF by Targeting PTEN Directly in Liver Cancer Cells

(A) Bioinformatics analysis: miR675 targets for human PTENα 3′ UTR. (B) pEZX-MT01-PTEN-3′-UTR luciferase activity assay is shown. Data are means of value from three independent experiments, mean ± SEM. **p < 0.01. (C) Western blotting analysis using anti-PTEN in Hep3B cells infected with rLV and rLV-miR372, respectively, is shown. β-actin as internal control is shown. (D) RT-PCR analysis using CTCF primers in Hep3B cells transfected with pcDNA3 and pcDNA3-PTEN, respectively, is shown. β-actin as internal control is shown. (E) Western blotting analysis using anti-CTCF in Hep3B cells transfected with pcDNA3 and pcDNA3-PTEN, respectively, is shown. β-actin as internal control is shown. (F) Western blotting analysis using anti-CTCF in Hep3B cell lines, including rLV, rLV-miR372, and rLV-miR372 plus transfected pcDNA3-PTEN, is shown.