Analysis of the Generated Synthetic TE mRNA and Cy3-Labeled TE mRNA by 1% Agarose Gel Electrophoresis
(Lane 1) RNA marker, 400 ng of (lane 2) Cy3-labeled TE mRNA, (lane 3) modified, or (lane 4) unmodified TE mRNA was loaded on 1% agarose gel. A single band at around 2,500 bases confirmed the purity and specific length of the synthetic mRNA. First, (A) Cy3-labeled TE mRNA was detected using an UV-transilluminator, and then (B) all nucleic acids were detected by GelRed staining.