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. 2018 Apr 19;11:518–527. doi: 10.1016/j.omtn.2018.04.004

Figure 1.

Figure 1

DRR Expression Is Reduced following Transfection of DRR+ U-251 MG Cells with DRR Antisense Oligonucleotides

All oligonucleotide strands were delivered at a concentration of 25 nM for 24 hr, and DRR expression was measured 72 hr post-transfection. (A) Oligonucleotide sequences used were as follows: all antisense strands comprise a phosphorothioate backbone, whereas all sense strands are synthesized with a phosphodiester backbone. Red, FANA; 5MeC, 2′-deoxy-5-methyl-cytidine. (B) DRR expression following treatment with single-stranded DRR AON sequences normalized to untreated control is shown. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test compared to Scrambled group (data are shown as mean + SD; n = 3; *p < 0.05; **p < 0.01). (C) DRR expression following treatment with double-stranded anti-DRR oligonucleotide sequences normalized to untreated control is shown. Data were analyzed using unpaired t test with Welch’s correction (data are shown as mean + SD; n ≥ 4; ***p < 0.001). (D) Representative western blot showing single-stranded and double-stranded antisense oligonucleotide DRR knockdown is shown.