Figure 1.

miR-199a-3p Mimics Controlled Liver Tumor Growth

(A) Mice were treated i.p. with the carcinogen N-diethylnitrosamine (DEN) at 10 days of age. miR-199a-3p mimics (5 mg/kg) were intraperitoneally injected three times a week for 3 weeks in 3-month-old mice. Identical schedule and amounts of scrambled oligonucleotides were used as negative controls (CTRL). Sorafenib treatment (5 mg/kg, daily oral administration) was used as a positive control for a reference drug in use against liver cancer. (B and C) At 5 months of age, mice were sacrificed, and liver lesions quantified. Number and size of nodules were significantly smaller in mice treated with miR-199a-3p and sorafenib than in the controls. (D) A similar experiment with a larger group of miR-199a-3p-treated mice and a slightly different timing of therapeutic interventions. Treatments began at 5 months of age, and mice were sacrificed immediately after the end of treatment. (E and F) In agreement with the results of the previous experiment, number and size of nodules were significantly smaller in mice treated with miR-199a-3p and sorafenib compared to the controls. In the figure, miR-199a-3p is indicated as miR-199.