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. 2018 Jun 1;9:722. doi: 10.3389/fpls.2018.00722

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Copyright © 2018 Li, Li, Yan, Liu, Zhang, Gao and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of NRT2 proteins. The amino acid sequences were aligned using ClustalW software and the phylogeny constructed using the neighbor-joining method with 1000 replicates through MEGA7 software. Database accession numbers of the amino acid sequences used are: Cucumis sativus CsNRT2.1 (MH213459), CsNRT2.3 (NP_001295862); Arabidopsis thaliana AtNRT2.1 (NP_172288), AtNRT2.2 (NP_172289), AtNRT2.3 (NP_200886), AtNRT2.4 (NP_200885), AtNRT2.5 (NP_172754), AtNRT2.6 (NP_190092), AtNRT2.7 (NP_196961); Populus trichocarpa PtNRT2.4A (POPTR_0009s01410.1), PtNRT2.4B (POPTR_0143s00200.1), PtNRT2.4C (POPTR_0009s01420.1), PtNRT2.5A (POPTR_0015s09290.1), PtNRT2.5B (POPTR_0015s09310.1); Vitis vinifera VvNRT2.4A (VIT_06s0061g00320), VvNRT2.4B (VIT_08s0040g01500), VvNRT2.5 (VIT_01s0127g00070), VvNRT2.7 (VIT_14s0066g00850); Sorghum bicolor SbNRT2.1 (Sb04g001000.1), SbNRT2.2 (Sb04g000990.1), SbNRT2.3 (Sb04g000970.1), SbNRT2.5 (Sb03g032310.1); Solanum lycopersicum SlNRT2.1 (AAF00053), SlNRT2.2 (NP_001266263), SlNRT2.3 (NP_001234127), SlNRT2.4 (XP_004240585), SlNRT2.7 (XP_004233327); Glycine max CmNRT2.1 (NP_001236444), CmNRT2.4 (XP_003539195); Hordeum vulgare HvNRT2.1 (AAC49531), HvNRT2.2 (AAC49532), HvNRT2.3 (AAD28363), HvNRT2.4 (AAD28364), HvNRT2.5 (ABG20828), HvNRT2.6 (ABG20829); Zea mays ZmNRT2.1 (AAN05088), ZmNRT2.2 (AAN05088), ZmNRT2.3 (XP_008645163), ZmNRT2.5 (XP_008656795); Brachypodium distachyon BdNRT2.1 (XP_003572550.1), BdNRT2.2 (XP_003572454.1), BdNRT2.3 (XP_003572590.2), BdNRT2.4 (XP_003570801.1), BdNRT2.5 (XP_003569637.1), BdNRT2.7 (XP_003566766.2); Oryza sativa OsNRT2.1 (AB008519), OsNRT2.2 (AK109733), OsNRT2.3a (AK109776), OsNRT2.3b (AK072215), OsNRT2.4 (LOC_Os01g3672); Vitis vinifera VvNRT2.1 (XP_002277127); Nicotiana plumbaginifolia NpNRT2.1 (CAA69387).