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Construction of CsNRT2.1-RNAi T2 generation cucumber lines. (A) The schematic diagram of CsNRT2.1-RNAi construct. (B) The relative expression analyses of CsNRT2.1 in WT, CsNRT2.1-RNAi lines grown on 0.5 hydroponic conditions by qRT-PCR. UBI in cucumber was used as an internal control. Error bars represent SE of three technical replicates of five biological replicates.
