Skip to main content
. 2018 May 1;8(5):866–878.

Figure 5.

Figure 5

Overexpression of SOX5 rescues loss of self-renewal and motile ability of silencing YAP1 cells. A. qRT-PCR analysis of YAP1 knockdown A549 cells with stably overexpressed SOX5 (shYAP1&OESOX5). B. Western blot showing the protein expressions of NANOG, VIMENTIN, YAP1 and SOX5 after overexpression of SOX5 in shYAP1 A549 cells. C. Tumorsphere formation assay showed that up-regulated SOX5 in shYAP1 A549 cells increased the volume (left panel) (scale bar represents 100 µm) and the number (right panel) of tumorspheres compared to shYAP1 group. D. The transwell invasion assay was performed to evaluate the invasion ability of shYAP1&OESOX5 group compared to control group (scale bar represents 50 µm). E. The wound healing assay was performed to evaluate the migration ability of shYAP1&OESOX5 group compared to control group, cells were photographed at 0 H and 24 H after wounding (magnifcation × 100). Data are shown as the mean ± SD. *indicates P<0.05, **indicates P<0.01.