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. 2018 May 1;8(5):835–851.

Figure 5.

Figure 5

Effects of HMGB2 on ovarian cancer cells growth and metastasis. A. Alteration of the HMGB2 expression levels of SKOV3 and OVCAR3 cells after transfection with the siHMGB2 or siCon. **P < 0.01 compared to control. B. SKOV3 and OVCAR3 cells were transfected with vector containing HMGB2 or control vector and the expression of HMGB2 was determined by qRT-PCR and immunoblotting. **P < 0.01 compared to control. C. MTT assay showed that transfection of the siHMGB2 attenuate the proliferation of ovarian cancer cells. D. The cells viability of HMGB2 over-expressing SKOV3 and OVCAR3 were assessed by MTT. E. Effect of HMGB2 down-expression on the colony growth of SKOV3 and OVCAR3 cells. **P < 0.01 compared to control. F. Effect of HMGB2 over-expression on the colony formation of ovarian cancer cells in vitro. **P < 0.01 compared to control. G. Wound closure assay was subjected to reveal that the CENPU inhibition hampered the cells mobility (left panel). Representative pictures of Transwell assays of SKOV3 and OVCAR3 cells after transfected with shCENPU or shCon (right panel). Scale bar: 200 μm. **P < 0.01 compared to control. H. In SKOV3 and OVCAR3 cells, over-expression of HMGB2 increased cell motility in wound healing analysis (left panel). Representative pictures of Transwell assays of SKOV3 and OVCAR3 cells after transfected with HMGB2 or control vector (right panel). Scale bar: 200 μm. **P < 0.01 compared to control. I. Representative images of H&E-stained lungs with metastases. Scale bar: 200 μm. **P < 0.01 compared to control. J. Representative images of H&E-stained lungs with metastases. Scale bar: 200 μm. **P < 0.01 compared to control.