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. 2018 May 1;8(5):835–851.

Figure 7.

Figure 7

Knock down of HMGB2 inhibits the growth and metastasis of ovarian cancer cells accelerated by HMGB2. A. CENPU over-expressing SKOV3 and OVCAR3 cells were transfected with siHMGB2. The mRNA levels of HMGB2 were analyzed by qRT-PCR and western blotting assays. B. After co-transfection of cells with siHMGB2 and CENPU, the levels of EMT-related proteins were measured by western blotting assay. C. HMGB2 down-expression counteracted the positive proliferative effects of CENPU over-expression in SKOV3 and OVCAR3 cells. D. HMGB2 inhibition hampered the colony formation of CENPU over-expression in ovarian cancer cells. E. CENPU over-expression SKOV3 or OVCAR3 cells were transfected with siHMGB2 and cell migration of ovarian cancer cells were analyzed by wound closure assay. F. Cell invasion in indicated SKOV3 or OVCAR3 cells were analyzed by Transwell assays. G. CENPU over-expressing cells were transfected with siHMGB2. Cells were injected into nude mice via tail vein. H&E staining of lung nodules. Scale bar: 200 μm. **P < 0.01 compared to vector, ##P < 0.01 compared to CENPU + siCon group.