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. 2018 Apr 23;7(5):bio033514. doi: 10.1242/bio.033514

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — iPSCs produce functional osteoblasts. (A) A mineralised matrix is deposited by iPSCs cultured in osteoinduction media for 21 days but not by cells cultured in non-osteoinductive media for the same time period. Positive hydroxyapatite staining detected under fluorescent light is shown in green. Positive staining for calcium deposition is shown in red for Alizarin Red S and black for von Kossa. Scale bars: 100 μm. Representative images from across all lines of iPSCs are shown. (B) Alkaline phosphatase activity is significantly upregulated following 21 days of differentiation of iPSCs on an OsteoAssay surface in osteogenic media. An unpaired Student's t-test was used to demonstrate statistically significant differences in the mean ALP values (*P<0.05).