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. 2018 May 15;10(5):1284–1294.

Figure 6.

Figure 6

Expression of Bax and Bcl-2 in SH-SY5Y cells. A. Cells were pretreated with MANF (400 ng/ml) for 2 h, then incubated with 2 mM MPP+ for 24 h. Immunoblot was applied to measure the proteins expression of Bax and Bcl-2. Values of densitometric analysis were calculated from three independent experiments. GAPDH was used as internal control. B. MPP+-induced increase of Bax/Bcl-2 was attenuated by MANF pretreatment. Data are presented as mean ± S.E.M. *P<0.05, **P<0.01 vs MPP+-treated group. #P<0.05, ##P<0.01 vs control group.