Figure 4.

Transwell assay of ATDC5 cells expressing empty vector, FLNB^WT, FLNB^L171R and FLNB^G1586R. The cells that migrated through the membrane and adhered to the lower surface of the membrane were fixed with 4% paraformaldehyde and stained with DAPI. For quantification, cells were counted under a microscope in four random fields. ATDC5 cells expressing FLNB^G1586R (a mutation associated with LS) presented a significantly decreased migration rates (D), compared with ATDC5 cells expressing empty vector (A), FLNB^WT (B), and FLNB^L171R (a mutation associated with BD) (C). (E) Bar plot of cell migration rates in different cell systems. Three experimental replicates were performed. Student’s t-test was used for the statistical analysis. All values were compared to Empty vector group. ns: not significant; ***: p-value < 0.001; **: p-value < 0.01; *: p-value < 0.05. Error bars indicate SD. WT: wild type; BD: boomerang dysplasia; LS: Larsen syndrome.