Fig. 2.

TCTP mRNA expression is developmentally regulated in T. brucei. (A) TCTP mRNA expression in BSF and PCF trypanosomes. (B,C) Northern blot analysis of TCTP1 and TCTP2 mRNA from BSF and PCF trypanosomes. The RNA was collected from cultures incubated with ActD (10 µg/ml) for indicated time (minutes). Ribosomal 18S RNA is used as a loading control. (D) Quantification of the relative RNA abundance of TCTP1 in PCF and TCTP2 in BSF trypanosomes. Three independent experiments were performed for each time point. Error bars represent standard deviation. Values are normalized to 18S rRNA. mRNA abundance in untreated cells was assigned as 100%. Half-lives were calculated to be 217 min in the PCF and 51 min in the BSF.