Fig. 6.

Rapamycin treatment prior to the onset of hamartoma formation has only a very minor effect on proliferation in Pten cKO retinas. (A-D′) Incorporation of BrdU in retinas from animals treated with rapamycin from P0-P7, including wild-type retinas in the centre (A,A′) and periphery (B,B′), and Pten cKO retinas in the central hamartoma (C,C′) and periphery (D,D’). Arrowheads mark BrdU⁺ cells. Blue is DAPI counterstain. (A′-D′) Desaturated red channel only. Asterisks indicate non-specific staining in blood vessels. (E) Quantitation of BrdU⁺ cells in the centre and periphery of wild-type (black bar) and Pten cKO (blue bar) retinas (wild-type rapamycin: n=6, Pten cKO rapamycin: n=8). gcl, ganglion cell layer; ham, hamartoma; inl, inner nuclear layer; onl, outer nuclear layer. Scale bars: 50 μm.