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. Author manuscript; available in PMC: 2018 Jun 8.
Published in final edited form as: J Biol Chem. 2005 Jul 1;280(35):31314–31324. doi: 10.1074/jbc.M503063200

Fig. 2. Truncated and mutant forms of HMGB1 exhibit differential capacity to supershift RAG-RSS complexes.

Fig. 2

Coexpressed MBP-RAG-1 and MBP-RAG-2 (RAG-1/2) was incubated with a radiolabeled intact 23-RSS substrate in the absence or presence of increasing amounts of the following forms of HMGB1 (as indicated above the gel): wild-type or tailless (A), tailless mtA or tailless mtB (B), basic (C), mtB (D), box A or shuffled (E), mtAB or mtA (F), box B′ (G), or box B (H). Protein-DNA complexes were fractionated by EMSA, and visualized from dried gels using a phosphorimager.