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. Author manuscript; available in PMC: 2018 Jun 8.
Published in final edited form as: J Biol Chem. 2005 Jul 1;280(35):31314–31324. doi: 10.1074/jbc.M503063200

Fig. 5. The acidic tail of HMGB1 helps enforce 12/23-regulated synapsis of RSS pairs by the RAG complex.

Fig. 5

A–D, RAG complex was incubated with radiolabeled intact 23-RSS in the absence or presence of various forms of HMGB1 (see Fig. 3 for amounts) with or without added cold 12- or 23-RSS partner DNA in the combinations indicated above the gel. Protein-DNA complexes were fractionated by EMSA and visualized using a phosphorimager.