Fig. 2.

Cells respond to SUV4-20 inhibition immediately and recover rapidly after removal of SUV4-20 inhibitor. a Heatmaps of the effect of SUV4-20 inhibition on the relative abundance of all discrete PTMs in SUM159 and MCF7 cells. b Relative abundance of discrete K20 methylation responds to SUV4-20 inhibition in SUM159 and MCF7 cells. c Volcano plot of changes in the relative abundance of proteoforms due to 12-h SUV4-20 inhibition in SUM159 cells (left panel) and MCF7 cells (right panel). Data points in the gray dashed squares indicate infinity fold change. d The level of H4{K20me2} recovers in 15 min after removal of SUV4-20 inhibitor in SUM159 cells. e Two selected decreased proteoforms in SUM159 cells, due to 2 h SUV4-20 inhibition, also recover in 15 min after removal of SUV4-20 inhibitor. *p < 0.05. Error bars in b, d, e represent standard error from three biological replicates