Fig. 4.

H4K20me2 and H4 acetylation are positively correlated on the single molecule level. a Hyperacetylated proteoforms with K20me2 increase, while hypoacetylated proteoforms with K20me2 decrease upon SUV4-20 inhibition in both cell lines. b The effects of SUV4-20 inhibition on binary combinations containing K20me2 and H4 acetylation in both cell lines. c The ternary combination of {K16ac, K20me2, K31ac} is increased in SUM159 cells upon SUV4-20 inhibition. d Volcano plot of proteoform changes in MCF7 cells after 15 min A-196 application. Data points in the gray square indicate infinity fold change. Error bars in a, b, c represent standard error from three biological replicates