Fig. 6.

HDAC inhibition diminishes the effects of SUV4-20 inhibition in typically prone SUM159 cells, while HATi facilitates SUV4-20 inhibition in typically resistant MCF7 cells. a Discrete H4 acetylations are increased rapidly upon HDAC inhibition. b Relative abundance of H4{K20me2} is not decreased in HDACi pretreated SUM159 cells. c Volcano plot of changes in proteoform abundance in SUM159 cells upon HDAC inhibition. Data points in the gray dashed squares indicate infinity fold change. d HDACi diminishes the effects of SUV4-20 inhibition on discrete H4{K20me2}. e The effect of HDACi then SUV4-20 inhibition on the abundance of <Nα-acK20me2> and <Nα-acK16acK20me2> in SUM159 cells. f Proteoform changes in response to the presence of HDACi then SUV4-20 inhibition in SUM159 cells. Data points in the gray square indicate infinity fold change. HATi facilitates SUV4-20 inhibition. g The effect of HATi then SUV4-20 inhibition on discrete H4K20 methylation states, as well as h two proteoforms (<Nα-acK20me2> and <Nα-acK16acK20me2>). *p < 0.05; **p < 0.01