Fig. 1.

Surface expression of chemokine receptors in CD20⁺ cells from PB and SF of RA and PsA patients. Mononuclear cells isolated from PB and SF labeled with two fluorochrome-conjugated mAbs and analyzed by flow cytometry. a Surface expression of chemokine receptors in CD20⁺ B cells from PB and SF of patients with RA (left) and PsA (right). Data represent mean ± SE of rMFI from six and five independent experiments, respectively. Expression level of each chemokine receptor in PB B cells considered 100%. b Surface expression of chemokine receptors in CD20⁺ B cells from PB and SF of patients with RA and PsA plotted together. Data represent mean ± SE of rMFI from 11 independent experiments. Representative flow cytometry histograms showing surface expression of CXCR4, CXCR5, CXCR7 and CCR6 in CD20⁺ cells from PB and SF of patients with RA. Dotted histograms represent isotype-matching control; dashed and closed histograms represent expression in B cells from PB and SF, respectively. *p < 0.05, **p < 0.01 by t test for paired samples. PB peripheral blood, PsA psoriatic arthritis, RA rheumatoid arthritis, SF synovial fluid, rMFI relative mean fluorescence intensity