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. 2018 Jun 7;20:114. doi: 10.1186/s13075-018-1611-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Surface expression of CXCR4, CXCR5, CXCR7 and CCR6 on memory (CD27⁺) and naïve (CD27^–) B cells from PB and SF of patients with arthritis. Mononuclear cells isolated from PB and SF labeled with a combination of three fluorochrome-conjugated mAbs and analyzed by flow cytometry. Surface expression of chemokine receptors studied in CD27⁺ and CD27^– in CD20⁺ cells from PB (considered 100%) and SF in patients with RA. Data expressed as mean ± SE of rMFI from seven independent experiments compared to PB expression of each chemokine receptor, considered 100%. *p < 0.05, **p < 0.01, ***p < 0.001 by t test for paired samples. rMFI relative mean fluorescence intensity, PB peripheral blood, SF synovial fluid