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. 2018 Jun 7;12:9. doi: 10.1186/s13036-018-0103-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Heterologous expression of SrcmPKS in S. lividans K4–114. a HPLC analysis of the extracts of S. lividans K4–114 harboring pSUN6 (i), pTZ3 (ii) and empty pRM5 vector (iii) respectively. Compounds were detected at 420 nm. b Formation of SEK15b (3) through spontaneous cyclization of the nascent decaketide chain