Table 2.
List of primers used in this studya
| Primers | Sequence |
|---|---|
| KS-F-PacI | 5’-AATTAATTAAGGAGGAGCCCATATGAGAAGGCGCGTCGTGGT-3’ |
| CLF-R-EcoRI-NheI | 5’-AAGAATTCGCTAGCCTACGGTCCCGACAGCGCCA-3’ |
| OxyA-5-PacI | 5’-AATTAATTAAGGAGGAGCCCATATGTCCAAGATCCATGACGC-3’ |
| OxyB-3-NheI | 5’-AAGCTAGCTCAGTCCCGGCCGCTGACCA-3’ |
| OxyC-5-SpeI | 5’-AAACTAGTGGAGGAGCCCATATGACCCTGCTCACCCTCTC-3’ |
| OxyC-3-NheI | 5’-AAGCTAGCTCACTTGTCCCGCGCGGCGC-3’ |
| ACP-F-SpeI | 5’-AAACTAGTGGAGGAGCCCATATGATGACGGTGGACGATCT-3’ |
| ACP-R-NheI | 5′- AAGCTAGCTCAGCCCACCCCGATGCCGT-3’ |
| 14–2-SARP-F-XbaI-NdeI | 5′- AATCTAGAGGAGGAGCCCATATGAGCTCCGACAGCGACTGCA-3’ |
| 14–2-SARP-R-XbaI | 5′- AATCTAGATCAGCCGGCCCGGCGCTGCC-3’ |
| PadRout-left-F-EcoRI | 5′- AAA GAATTC TACTCGCCGGAGTGCGCACGGTCGAA-3’ |
| PadRout-left-R-XbaI | 5′- AAA TCTAGA CTTCTTCGCGCAGCTTGCGCAGCT-3’ |
| PadRout-right-F-XbaI | 5′- AAA TCTAGA CGTACCGCTGTCGCCGCGAT-3’ |
| PadRout-right-R-HindIII | 5′- AAA AAGCTT ATGATCTCGATCATCGAGCG-3’ |
| PadRout-confirm-F | 5’-AACATATGGCTCTGGGCACGCTGCA-3’ |
| PadRout-confirm-R | 5’-AAGAATTCTCACTGCGGGTCCTCCTGCG-3’ |
aThe restriction sites are underlined