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. 2018 Mar 6;11:272–283. doi: 10.1016/j.omtn.2018.02.012

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

miR-107 Inhibits Apoptosis of Bovine Primary Myoblasts

(A and B) Bovine primary myoblasts were transfected with miR-107 mimic and inhibitor, and cell apoptosis was determined (A) and counted (B) by annexin V-FITC/PI binding followed by flow cytometry. (C–E) The mRNA expression of apoptosis marker genes Caspase9 (C), Caspase3 (D), and P53 (E) was detected by real-time qPCR. (F) The protein expression of apoptosis marker genes was detected by western blotting. Values are means ± SEM for three individuals. *p < 0.05.