Figure 5.
miR-107 Directly Targets Wnt3a Gene
(A) The binding sites analysis in Wnt3a 3′ UTR. (B) Cells were co-transfected with miR-107 mimic and psi-Wnt3a-3′ UTR-W/Mut, and Renilla luciferase activity was normalized to the firefly luciferase activity. (C) The mRNA expression Wnt3a was detected by real-time qPCR. (D) The protein expression of Wnt3a was detected by western blotting. Values are means ± SEM for three individuals. *p < 0.05.