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. 2017 Nov 24;11:323–336. doi: 10.1016/j.omtn.2017.11.009

Figure 2.

Figure 2

miR-208a-3p Inhibits Osteoblast Activity and Matrix Mineralization In Vitro

(A) Relative expression of miR-208a-3p in MC3T3-E1 cells after transfection with mock, antagomiR-NC, and antagomiR-208a-3p. (B) Relative ACVR1 mRNA level in MC3T3-E1 cells after transfection with mock antagomiR-NC and antagomiR-208a-3p. (C) Staining of calcium deposition by alizarin red in MC3T3-E1 cells after transfection with mock, antagomiR-NC, and antagomiR-208a-3p in osteogenic medium for 24 days. (D) ALP staining of MC3T3-E1 cells after transfection with mock, antagomiR-NC, and antagomiR-208a-3p for 24 hr. (E) Effects of antagomiR-208a-3p transfection on expression of osteoblast differentiation marker genes ALP, Col1a1, OCN, and Runx2. (F) Western blot examination of ACVR1 protein expression in osteoblasts was enhanced after antagomiR-208a-3p transfection. All data were expressed as means ± SD. Significance is noted at these thresholds: *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA with a post hoc test was performed. Statistical differences between two groups were determined by Student’s t test.