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. 2017 Nov 24;11:323–336. doi: 10.1016/j.omtn.2017.11.009

Figure 3.

Figure 3

miR-208a-3p Targets ACVR1 to Functionally Inhibit Osteoblast Activity In Vitro

(A) Effect of endogenous miR-208a-3p on the luciferase activity of WT ACVR1 3′ UTR (luc-UTR), ACVR1 3′ UTR mutant (luc-UTR-Mut), or the luciferase vector control (luc-vector) after treatment with antagomiR-208a-3p in MC3T3-E1 cells. (B) Effect of endogenous miR-208a-3p on the luciferase activity of WT ACVR1 3′ UTR (luc-UTR), the ACVR1 3′ UTR mutant (luc-UTR-Mut) after treatment with antagomiR-208a-3p or its negative control (antagomiR-NC) in MC3T3-E1 cells. (C–E) qPCR and western blot analysis of ALP (C), Col1a1 (D), and OCN (E) protein expression in MC3T3-E1 cells in which ACVR1 expression was inhibited by siRNA. All data were expressed as means ± SD. Significance is noted at these thresholds: *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA with a post hoc test was performed. Statistical differences between two groups were determined by Student’s t test. NS, not significant; NC, negative control; Rluc, Renilla luciferase.