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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: DNA Repair (Amst). 2018 Apr 17;66-67:1–10. doi: 10.1016/j.dnarep.2018.04.001

Fig. 5. Chromatin association of WT vs. non-acetylable mutant NEIL1 and analysis of their IP complexes.

Fig. 5

(A and B) Western analysis of different extracts of HEK293 cells expressing FLAG-tagged WT vs. (A) 3KR, or (B) 3KA, N1-311 mutant NEIL1 for FLAG (NEIL1) level.

(C) FLAG Co-IP analysis on the whole nuclear extracts containing chromatin fractions of FLAG-tagged WT vs. 3KR mutant NEIL1 expressing cells for different chromatin and BER/SSBR proteins.