(A and C) Western analysis of HEK293 and HT29 cell extracts for ectopic expression of (A) WT and 3KA, and (C) WT and 3KR mutant NEIL1, respectively after downregulation of endogenous NEIL1.
(B and D) Clonogenic cell survival assay was performed in these cells after treating with various doses of glucose oxidase. Details are described in Materials and Methods. * denotes p value < 0.05 (unpaired T Test; online Graphpad software).