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. Author manuscript; available in PMC: 2019 Mar 16.

Published in final edited form as: Methods Enzymol. 2018 Mar 16;604:45–88. doi: 10.1016/bs.mie.2018.01.035

Table 7.

Lysis Buffer Screen Formulation

Condition	Buffer	Additives
1	50 mM Tricine pH 8.5	500 mM NaCl, 10% glycerol
2	50 mM Tricine pH 8.5	50 mM (NH₄)₂SO₄, 10% glycerol
3	50 mM Tricine pH 8.5	50 mM NaCl, 5% DMSO
4	50 mM Tricine pH 8.5	50 mM NaCl, 5% ethanol
5	50 mM Tricine pH 8.5	50 mM NaCl, 1% Triton X-100
6	50 mM Tricine pH 8.5	50 mM NaCl, 100 mM urea
7	50 mM Tricine pH 8.5	50 mM NaCl, 50 mM Arg, 50 mM Glu
8	50 mM HEPES pH 7.0	50 mM (NH₄)₂SO₄, 10% glycerol
9	50 mM HEPES pH 7.0	50 mM NaCl, 5% DMSO
10	50 mM HEPES pH 7.0	50 mM NaCl, 5% ethanol
11	50 mM HEPES pH 7.0	50 mM NaCl, 1% Triton X-100
12	50 mM HEPES pH 7.0	50 mM NaCl, 100 mM urea
13	50 mM HEPES pH 7.0	50 mM NaCl, 50 mM Arg, 50 mM Glu
14	50 mM HEPES pH 7.0	500 mM NaCl, 10% glycerol