Table 2.

Summary of pre-steady-state kinetic parameters for incorporation of dNMPs by BST pol using different metal ions with 66 mM Tris-HCl (pH 7.3) at 23 °C.

Metal ion	dNTP	Template	kpol (s−1)	Kd,app (mM)	kpol/Kd,app (mM−1 s−1)
Mg2+	dTTP	dA	20	26	7.7 × 10−1
	dATP	dC	0.07	3000	2.3 × 10−5
	dTTP	dC	0.002	2600	7.5 × 10−7
Co2+	dTTP	dA	123	28	4.4
	dATP	dC	0.3	267	1.1 × 10−3
	dTTP	dC	0.003	354	8.5 × 10−6
Mn2+	dTTP	dA	166	22	7.5
	dATP	dC	9	293	3.1 × 10−2
	dTTP	dC	0.004	396	1.0 × 10−5
Cd2+	dTTP	dA	15	15	1
	dATP	dC	0.08	67	1.2 × 10−3
	dTTP	dC	0.0005	174	2.9 × 10−6

Standard deviations were within 10–20% and ~30% for k_pol and K_d,app values, respectively.