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Equilibrium fluorescence titration data for BST pol using Mg2+, Co2+, Mn2+, and Cd2+ with 2AP as the templating base.
| Metal ion | P/T | dNTP | Kd,g (μM) |
|---|---|---|---|
| Mg2+ | (ddP/T) | dTTP | 1 |
| Mg2+ | (ddP/T) | dCTP | 1463 |
| Co2+ | (ddP/T) | dTTP | 3 |
| Co2+ | (ddP/T) | dCTP | 25 |
| Mn2+ | (ddP/T) | dTTP | 0.1 |
| Mn2+ | (ddP/T) | dCTP | 0.3 |
| Cd2+ | (ddP/T) | dTTP | 0.4 |
| Cd2+ | (ddP/T) | dCTP | ND |
The concentrations of Mg2+, Co2+, Mn2+, and Cd2+ used in the titrations were 10 mM each. Additional details are provided in the text. ND = not determined.
