Table 4.

Pre-steady-state kinetic parameters for extension beyond dA/dC mismatch for BST pol using Mg²⁺, Mn²⁺, Co²⁺ and Cd²⁺ where dAMP is being inserted opposite a templating dT.

Metal ion	kpol (s−1)	Kd,app (μM)	kpol/Kd,app (μM−1 s−1)
Mg2+	0.1	618	1.6 × 10−4
Co2+	1	1100	9.1 × 10−4
Mn2+	2	127	1.6 × 10−2
Cd2+			2.3 × 10−4a

^aRepresents the ratio of the k_pol to the K_d,app value as saturation is not achieved with the incoming dNTP.

Standard deviations were within 10–20% and ~30% for k_pol and K_d,app values, respectively.