Table 2.

Plasmids constructed in this study

Plasmid	E. coli Promoter	Gene under control of the cloned promoter
pZXD44	T7A1/O4	tet^a
pZXD50	tac	tet^a
pZXD49	lacUV5	tet^a
pZXD47	lac	tet^a
pZXD48	lacL8	tet^a
pZXD57	T7A1/O4	GFPuv^b
pZXD58	tac	GFPuv^b
pZXD56	lacUV5	GFPuv^b
pZXD55	lac	GFPuv^b
pZXD54	lacL8	GFPuv^b
pZXD59	T7A1/O4	Reverse GFPuv^b
pZXD60	T7A1/O4	lacZ^c
pZXD60A	T7A1/O4	N/A^d
pZXD61	T7A1/O4	Reverse lacZ^c
pZXD62	T7A1/O4	GFPuv^b
pZXD63	T7A1/O4	tet^a
pZXD63A	T7A1/O4	N/A^d

The tetracycline resistance gene of pBR322 (tet) encodes a 41 kD transmembrane protein TetA.

The green fluorescence protein UV (GFPuv) gene encodes a cytosolic protein GFPuv.

The lacZ gene encodes a cytosolic protein β-D-galactosidase.

N/A represents not applicable. Plasmid pZXD60A and 63A have an amber mutation in the start codon of lacZ and tet, respectively.