Fig. 2.

Detailed analysis of high dose morphine-induced behavioral changes. Six distinct animal behavior (A. Moving time, B. distance, C. rearing time, D. clockwise (CW) rotation time, E. Counter-clockwise (CCW) rotation time, and F. the ratio of distance travelled in periphery vs center were recorded after a single-dose of morphine (10 mg/kg, s.c.) in 8 and 24 week old rats and were analyzed using ActiMot software (TSE Systems). Measurements were taken prior to drug administration (basal), 30 and 180 min post-administration, as described in Section 2. Differences between the 30 min time point and the basal or 180 min time points were significantly different (*p<0.05, ***p<0.001 and ****p<0.0001, using repeated measure one-way ANOVA with Sidak's multiple comparison post hoc test). Likewise, significant differences against 8 week old animals were observed for some time points (^##p<0.01 and ^###p<0.001, using non-repeated measure one-way ANOVA with Sidak's multiple comparison post hoc test). All data are presented as mean±SEM, n=5 per group.