Figure 2.

Absence of CD11b leads to more severe granuloma formation and hyper-recruitment of inflammatory cells in vivo. (a–d) WT and CD11b^−/− mice (n=8) were intravenously injected with 3.75 mg kg⁻¹ TDM in an oil-in-water emulsion and killed at 7 days post-TDM challenge. (a) Lungs were isolated and weighed to calculate the lung weight index (LWI). (b) Lungs were stained with hematoxylin and eosin (H&E) for histology analysis. Scale bars, 100 mm. (c) Flow cytometry was performed for leukocyte subset analysis with distinct markers for monocytes and macrophages (Mo/Ma; CD11b+ Ly6G−), neutrophils (PMN; CD11b+ Ly6G+), T cells (CD3+) and B cells (CD19+). (d) The lung homogenates were analyzed by ELISA for IL-6 and TNF-α production. (e, f) For the air pouch model, mice (n=8) were dorsolaterally injected with sterile air on days 0 and 3 followed by injection of 2.5 mg kg⁻¹ of a TDM emulsion on day 7. On day 8, wash fluid from the pouches was assessed by flow cytometry for leukocyte subsets, as described above (e), and the IL-6 and TNF-α cytokine levels were determined by ELISA (f). *P<0.05, **P<0.01, ***P<0.0001 (two-tailed unpaired Student’s t-test). Data are representative of two independent experiments. (b–f, mean and s.d. of eight mice per group.)