Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 5;50(2):e439. doi: 10.1038/emm.2017.256

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

ITIM-containing SIRPα is critical for SHP1 docking and interaction with Syk. (a) iBMM cells were transfected with Flag-CD11b, Flag-Syk, V5-Mincle, HA-Lyn or Flag-Shp1 for 24 h and then treated with TDM for 24 h before assessment by PLA assay. Interactions between endogenous SIRPα and the transfected proteins were visualized as fluorescent spots (red, PLA signal). Nuclei were stained with DAPI (blue). The number of PLA signals was determined for at least 50 cells for each condition. (b) Knockdown of SIRPα was confirmed by immunoblot (top panel). Culture media was collected after 24 h of TDM stimulation and subjected to ELISA for measurement of IL-6 and TNF-α production (bottom panel). (c) WT and SIRPα-deficient iBMMs were stimulated with TDM for the indicated times, and activation of Syk and Erk was determined by immunoblot. β-Actin protein expression was used as a loading control. Data are representative of two (c) or three (a, b) independent experiments. *P<0.05, ***P<0.0001 (two-tailed unpaired Student’s t-test).