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. 2018 Jun 5;6:e4902. doi: 10.7717/peerj.4902

Table 1. Details on primer pairs for species identification in fur samples.

Primer set Target mtDNA Forward (5′-3′) Reverse (5′-3′) Amplicon size (bp) Primer positions Ta (°C) Needs sequencing
furND1 ND1 CTAGCAATTATTCTCCTATCAGTCC ACTAGTTCGGATTCTCCTTCAGT 187 3,183–3,370 U966391 53 YES
lupCR CR GTGCTATGTCAGTATCTCCAGGT GAAAGATCAGTGAATTATGAGATTGAG 149 15,494–15,643 U966391 56 NO
nycCR CR ATACCTCACCACCCTCTATTT TGATTAAGTAGTTTATGTCTATTGTGAC 155 15,468–15,623 NC0137002 52 NO
vulCR CR ATTAACCAGTGTGTTACGACTATCC TGCAGTCATGTATGCTCCTGATA 169 15,469–15,638 AM1810373 56 NO
proCR CR CCATCGCATACTCAATCCTACTG TCTGCGACGTAGGCCTATTTACTA 173 1,574–1,747 AB2978044 53 NO
felCytb cytb CCTAGTAGCGGATCTCCTAACC ACGGTTTTCAATAATGCCTGAG 145 16,019–16,164 U207535 55 YES

Notes:

Amplicon sizes include primer lengths. bp, base pair; Ta, annealing temperature; ND1, NADH ubiquinone oxidoreductase subunit ND1 gene; CR, control region, cytb, cytochrome b. Primer positions according to reference complete mitochondrial sequences.

2

published only in database;

4

published only in database;