Table 1. Details on primer pairs for species identification in fur samples.
Primer set | Target mtDNA | Forward (5′-3′) | Reverse (5′-3′) | Amplicon size (bp) | Primer positions | Ta (°C) | Needs sequencing |
---|---|---|---|---|---|---|---|
furND1 | ND1 | CTAGCAATTATTCTCCTATCAGTCC | ACTAGTTCGGATTCTCCTTCAGT | 187 | 3,183–3,370 U966391 | 53 | YES |
lupCR | CR | GTGCTATGTCAGTATCTCCAGGT | GAAAGATCAGTGAATTATGAGATTGAG | 149 | 15,494–15,643 U966391 | 56 | NO |
nycCR | CR | ATACCTCACCACCCTCTATTT | TGATTAAGTAGTTTATGTCTATTGTGAC | 155 | 15,468–15,623 NC0137002 | 52 | NO |
vulCR | CR | ATTAACCAGTGTGTTACGACTATCC | TGCAGTCATGTATGCTCCTGATA | 169 | 15,469–15,638 AM1810373 | 56 | NO |
proCR | CR | CCATCGCATACTCAATCCTACTG | TCTGCGACGTAGGCCTATTTACTA | 173 | 1,574–1,747 AB2978044 | 53 | NO |
felCytb | cytb | CCTAGTAGCGGATCTCCTAACC | ACGGTTTTCAATAATGCCTGAG | 145 | 16,019–16,164 U207535 | 55 | YES |
Notes:
Amplicon sizes include primer lengths. bp, base pair; Ta, annealing temperature; ND1, NADH ubiquinone oxidoreductase subunit ND1 gene; CR, control region, cytb, cytochrome b. Primer positions according to reference complete mitochondrial sequences.
published only in database;
published only in database;