Table 2. Presence (YES) or absence (NO) of amplification products in the reference species using non-specific and specific primer sets.
| Family | Species | Primer set | |||||
|---|---|---|---|---|---|---|---|
| furND1 | lupCR | nycCR | vulCR | proCR | felCytb | ||
| Canidae | Canis lupus ssp. (dog/wolf) | YES (0.091) |
YES (0.103) |
NO | NO | NO | NO |
| Canidae | Canis latrans (coyote) | YES (0.011) |
NO | NO | NO | NO | NO |
| Canidae | Canis aureus (jackal) | YES (0.120) |
NO | NO | NO | NO | NO |
| Canidae | Nyctereutes procyonoides (raccoon dog) | YES (0.048) |
NO | YES (0.025) |
NO | NO | NO |
| Canidae | Vulpes vulpes (red fox) | YES (0.105) |
NO | NO | YES (0.110) |
NO | NO |
| Felidae | Felis silvestris ssp. (domestic/wild cat) | YES (0.045) |
NO | NO | NO | NO | YES (0.108) |
| Felidae | Lynx lynx (Eurasian lynx) | YES (50) |
NO | NO | NO | NO | YES (49.8) |
| Felidae | Panthera tigris (tiger) | YES (2) |
NO | NO | NO | NO | YES (19.8) |
| Procyonidae | Procyon lotor (raccoon) | NO | NO | NO | NO | YES (1.1) |
NO |
| Mustelidae | Martes foina (beech marten) | YES | NO | NO | NO | NO | NO |
| Mustelidae | Lutra lutra (otter) | YES | NO | NO | NO | NO | NO |
| Leporidae | Oryctolagus cuniculus (rabbit) | Aspecific bands | NO | NO | NO | NO | NO |
| Leporidae | Lepus europaeus (brown hare) | NO | NO | NO | NO | NO | NO |
| Sciuridae | Sciurus vulgaris (red squirrel) | YES | NO | NO | NO | NO | NO |
| Hominidae | Homo sapiens (human) | NO | NO | NO | NO | NO | NO |
Note:
Values of LOD (limit of detection) DNA in picograms (pg) are in parentheses. LODs were obtained only in canid and felid species using furND1 primer pair and in the target species using the other primer sets.