Figure 3. Regulation of the Bcl-2 family proteins during F5-induced apoptosis.

(A) MCF7 cells were treated with F5 for 72 h at IC50, comparing to the control (Lane − vs. Lane +). The expression of BID, Bcl-2, Bax, and β-actin in the cell lysates were shown. Molecular weight of the standards (kDa) are indicated on the most left of the first panel from the left. Coomassie blue staining of PVDF membrane (50 μg protein lysates/lane) is shown in the second panel from the left. (B) Relative expression of Bcl-2 family proteins after normalization to the control (untreated cells) which was set at 1. Data expressed as mean ± SD (n = 2) where p < 0.05 in untreated cells (negative control) vs. F5-treated MCF7 cells.