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. Author manuscript; available in PMC: 2018 Aug 18.
Published in final edited form as: Methods Enzymol. 2017 Aug 18;597:83–141. doi: 10.1016/bs.mie.2017.06.029

Figure 5.

Figure 5

Conjugation of puromycin-containing oligonucleotide to mRNA by photocrosslinking between psoralen and uracil. (A) Structure of the psoralen moiety connected to oligonucleotide containing puromycin. (B) Structure of the photo-crosslinked psoralen moiety with two uracil adducts. (C) Overview of the psoralen-mediated photo-crosslinking of puromycin-containing oligonucleotides and mRNA at the end of the open reading frame.